Fundamental Sciences
Codon Genomics is actively involved in applying new sequencing and omics technology to basic and fundamental sciences. We agree that the basic research serves as a important foundation and breakthrough to the development of more practical applications:
What we do
By leveraging the NGS technology and bisulfite-induced selective deamination of unmethylated cytosine to uracil, cysosine methylation which caused the temporal and spatial gene expression and chromatin remodeling can be detected.
Direct genetic analysis of massive genomes and identify the microbiome present in an environmental sample, bypassing the resources-intensive isolation and subculture of individual microbes, and problem of high percentage of unculturable microbes.
Simultaneous profiling of transcriptomes of host and pathogen during the infection stage, and uses in silico analysis to distinguish species-specific transcripts to infer the molecular interactions.
The use of third generating sequencing technology with extremely long read length (up to 20-30 kb), the full length transcripts can be sequenced enabling the study of alternative splicing events and transcriptional regulation which are prevalent in higher eukaryotic organisms like humans.
Small non-coding RNAs such as microRNA (miRNA0, small interfering RNA (siRNA) and piwi interacting RNA (piRNA) act in gene silencing and post-transcriptional regulation of gene expression. By generating small RNA sequencing library and sequenced using NGS technology, novel biomarkers (small RNAs which regulate gene expression post-transcriptionally) contribute to specific phenotype can be determined.
Our achievements
Chow, Y.P., Murad, N.A.A., Rani, Z.M., Khoo, J.S., Chong, P.S., Wu, L.L. and Jamal, R., 2017. Exome sequencing identifies SLC26A4, GJB2, SCARB2 and DUOX2 mutations in 2 siblings with Pendred syndrome in a Malaysian family. Orphanet journal of rare diseases, 12(1), p.40.
Wong, Y.C., El Ghany, M.A., Naeem, R., Lee, K.W., Tan, Y.C., Pain, A. and Nathan, S., 2016. Candidate essential genes in Burkholderia cenocepacia J2315 identified by genome-wide TraDIS. Frontiers in microbiology, 7.
Chin, C.Y., Hara, Y., Ghazali, A.K., Yap, S.J., Kong, C., Wong, Y.C., Rozali, N., Koh, S.F., Hoh, C.C., Puthucheary, S.D. and Nathan, S., 2015. Global transcriptional analysis of Burkholderia pseudomallei high and low biofilm producers reveals insights into biofilm production and virulence. BMC genomics, 16(1), p.471.
Ho, C.L., Tan, Y.C., Yeoh, K.A., Ghazali, A.K., Yee, W.Y. and Hoh, C.C., 2016. De novo transcriptome analyses of host-fungal interactions in oil palm (Elaeis guineensis Jacq.). BMC genomics, 17(1), p.66.
Kuan, C.S., Chan, C.L., Yew, S.M., Toh, Y.F., Khoo, J.S., Chong, J., Lee, K.W., Tan, Y.C., Yee, W.Y., Ngeow, Y.F. and Ng, K.P., 2015. Genome analysis of the first extensively drug-resistant (XDR) Mycobacterium tuberculosis in Malaysia provides insights into the genetic basis of its biology and drug resistance. PloS one, 10(6), p.e0131694.